Catalog No.: 100970

Net Contents: 1 x 15.0 mg AggRecetin (Ristocetin)

1 x 2.0 mL Diluent

AGGRECETIN® 10 - 15mg Ristocetin

AggRecetin®: Improving Platelet Function Results through Ristocetin Cofactor Activity Testing

AggRecetin® (Ristocetin) is the required reagent, used in combination with other IVD components, to perform a Ristocetin Cofactor Activity test on Platelet Rich Plasma

Mediates Platelet Adhesion (Agglutination) by Binding to Collagen and Platelets
Causes vWF to Bind to the Gp1B Platelet Receptor
Used with Patient Platelet Poor Plasma and Fixed Platelets to Perform the Ristocetin Cofactor Activity Assay
Determines the Presence and Integrity of the Platelet Glycoprotein (GP) Ibα-V-IX Complex and von Willebrand Factor (VWF) Interaction when Performed Using Platelet Rich Plasma
Causes Ristocetin Induced Platelet Aggregation (RIPA) in Platelet Rich Plasma Samples when AggRecetin is Added in Two Concentrations

AggRecetin® (Ristocetin) emerges as a lyophilized manifestation of Ristocetin A Sulfate, a compound with an enigmatic chemical structure extracted from Nocardia lurida. The composition of AggRecetin is particularly noteworthy, containing an impressive concentration of over 90% Ristocetin A, thereby affirming its status as a substance of exceptional purity and potency.

The functionality of AggRecetin (Ristocetin) within the complex orchestration of platelet dynamics is equally remarkable. In the intricate interplay between Ristocetin and the von Willebrand factor, a captivating phenomenon unfolds. This substance has the unique ability to induce the agglutination of platelets, a process observed in both platelet-rich plasma and standardized fixed platelet suspensions.

Delving into the molecular ballet that occurs in the presence of Ristocetin, it becomes evident that this substance serves as a catalyst, prompting the intriguing interplay between platelets and von Willebrand factor. The agglutination witnessed in platelet-rich plasma or standardized fixed platelet suspensions not only underscores the potency of AggRecetin (Ristocetin) but also offers a nuanced glimpse into the complex mechanisms governing platelet behavior. This revelation holds particular significance in the broader context of hemostasis and coagulation, contributing to our understanding of the intricacies that regulate these vital physiological processes. AggRecetin, with its high concentration of Ristocetin A, thus emerges not merely as a reagent but as a key to unraveling the mysteries of platelet function and the intricate web of interactions that define hemostatic balance.

Explore Product Data Sheet

Discover Expected Results Chart

Please delve into the literature provided on this page, including the AggRecetin Reagent Product Data Sheet, Routine Agonist Expected Results Chart, and the AggRecetin Instruction for Use (IFU), along with the AggRecetin Safety Data Sheet (SDS) — available in six languages. Furthermore, for our European and United Kingdom customers and distributors, examine the AggRecetin EU Declaration of Conformity and the AggRecetin UK Declaration of Conformity to ensure compliance and understanding tailored specifically for these regions. This information is essential for a thorough understanding of the product details and specifications.

Bio/Data Corporation’s Aggregation Reagents: Accessible Safety Data Sheets for Secure Handling

Bio/Data Corporation provides comprehensive Safety Data Sheets (SDS) for each of its carefully formulated aggregation reagents. These essential documents ensure safe handling, storage, and spill management while offering clear instructions on potential hazards. Designed for easy viewing, saving, and printing, the SDS are readily accessible to support a safe work environment and responsible chemical management.

Access Safety Data Sheet

Product Description

AggRecetin® Reagent is Ristocetin A Sulfate, a glycopeptide of unknown chemical structure which has been isolated from Nocardia lurida. Ristocetin contains in excess of 90% Ristocetin A. AggRecetin (Ristocetin) Reagent has been optimized for use with Light Transmission Aggregometers. It may also be used with other turbidometric or impedance analyzers, and flow cytometers.

Review Instructions For Use

Intended Purpose

AggRecetin (Ristocetin) Reagent to perform Ristocetin induced platelet aggregation (RIPA) or Ristocetin Cofactor Activity Assays on a Platelet Rich Plasma (PRP) test sample.

Detection / Measurement

AggRecetin (Ristocetin) Reagent is used, in conjunction with other diluents and control samples, to measure changes of the light transmission in a Platelet Rich Plasma (PRP) test sample.

Product Function

AggRecetin (Ristocetin) Reagent provides insight into different aspects of platelet function / quality. This Reagent aids in accessing various acquired and inherited platelet disorders or the efficacy of anti-platelet therapies.

Test Principle

When introduced to a stirred, 37°C Platelet Rich Plasma (PRP) test sample, exogenous Reagents such as Ristocetin stimulate platelets, prompting them to undergo shape change and aggregate. This initial aggregation is called primary aggregation and is reversible. However, normal platelets possess the ability to release endogenous ADP from their granules, leading to a secondary, irreversible wave of aggregation. For Ristocetin Cofactor Activity Assays, It induces passive platelet agglutination in the presence of von Willebrand Factor. The Light Transmission Platelet Aggregometer effectively captures these changes by displaying parameters such as lag phase, shape change, and the rate and extent of aggregation over a predetermined testing period.

READ MORE... Easily Download the AggRecetin Reagent Instructions for Use in Your Selected Language.

Reagents Provided

AggRecetin Reagent, 1 x 15 mg.

AggRecetin Diluent, 1 x 2.0 mL.

Reagents and Materials Required but Not Provided

Purified Water (Distilled, Deionized, Reagent Grade), pH 5.3 – 7.2 for reconstitution
TRIS Buffered Saline (TBS) or 0.85% physiologic saline for dilutions

NOTE: USING BLOOD BANK SALINE WILL CAUSE ERRONEOUS RESULTS.

Materials and Accessories

Platelet Aggregometer
Centrifuge
Electronic Pipette
Pipette Tips
Aggregometer Test Tubes (Siliconized)
Aggregometer Stir Bars (Plastic Coated)
Plastic Sample Tubes and Caps (For Dilutions)

Reagent Storage

AggRecetin Reagent does not require temperature protection during shipment.
Upon receipt, store AggRecetin Reagent at 2 – 8° C in its original packaging.
Reconstituted AggRecetin Reagent is stable for 7 days when stored in its’ tightly capped, original container at 2 – 8° C.

AggRecetin Ristocetin Aggregation Reagent

AGGRECETIN IS AVAILABLE

IN TWO SIZES

AggRecetin Ristocetin Reagent vW Cofactor Results

Specific Information Provided

AggRecetin Reagent is not intended for the detection of a specific disorder, condition, or risk factor. AggRecetin (Ristocetin) Reagent plays a pivotal role in assessing the functional / quality activity of von Willebrand Factor (vWF) through the vWF Cofactor Assay, a critical test for evaluating vWF activity and function. This assay relies on AggReccetin (Ristocetin) to induce the interaction between vWF and platelets, under shear stress conditions. By binding to vWF, AggRecetin (Ristocetin) triggers a conformational change in the protein, exposing binding sites for platelet glycoprotein Ib (GPIb), facilitating platelet adhesion and aggregation. The Ristocetin Cofactor activity of vWF, observed in the presence of AggRecetin (Ristocetin) Reagent, evaluates its ability to promote the agglutination of formalin-fixed or fresh platelets in a patient’s plasma. A decrease in Ristocetin Cofactor activity indicates an impairment or dysfunction of vWF, characteristic of von Willebrand Disease (vWD) or related disorders, by assessing the extent of platelet aggregation in response to Ristocetin.

Bio/Data Corporation's Commitment to IVDR Compliance
Bio/Data Corporation proudly complies with the stringent EU In Vitro Diagnostic Regulation (IVDR) 2017/746 and other relevant legislation. Through its Declaration of Conformity (DOC), the company ensures that its devices meet essential health, safety, and environmental standards.

Bio/Data Corporation's Commitment to UKCA Compliance
Bio/Data Corporation ensures product excellence by strictly adhering to UKCA regulations. The company's Declaration of Conformity (DOC) affirms its dedication to meeting health, safety, and environmental standards outlined in UKCA directives and any related updates, demonstrating its unwavering commitment to compliance.

View EU Declaration of Conformity

View UK Declaration of Conformity

AGGRECETIN® 10 - 15mg Ristocetin

AggRecetin®: Improving Platelet Function Results through Ristocetin Cofactor Activity Testing

AggRecetin® (Ristocetin) is the required reagent, used in combination with other IVD components, to perform a Ristocetin Cofactor Activity test on Platelet Rich Plasma

​

Mediates Platelet Adhesion (Agglutination) by Binding to Collagen and Platelets

Causes vWF to Bind to the Gp1B Platelet Receptor

Used with Patient Platelet Poor Plasma and Fixed Platelets to Perform the Ristocetin Cofactor Activity Assay

Determines the Presence and Integrity of the Platelet Glycoprotein (GP) Ibα-V-IX Complex and von Willebrand Factor (VWF) Interaction when Performed Using Platelet Rich Plasma

Causes Ristocetin Induced Platelet Aggregation (RIPA) in Platelet Rich Plasma Samples when AggRecetin is Added in Two Concentrations